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KMID : 1134820140430050705
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Journal of the Korean Society of Food Science and Nutrition
2014 Volume.43 No. 5 p.705 ~ p.711
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Hypopigmenting Effects of Extracts from Bulbs of Lilium Oriental Hybrid ‘Siberia’ in Murine B16/F10 Melanoma Cells
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Yoon Hoon-Seok
Yang Kyong-Wol
Kim Jung-Eun
Kim Jeong-Mi
Lee Nam-Ho
Hyun Chang-Gu
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Abstract
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In order to develop a skin-whitening agent, melanin contents and intracellular tyrosinase activity were determined by western blotting. Ethyl acetate fractions of 80% ethanol extracts from lily (Lilium Oriental Hybrid ‘Siberia’) bulbs (R-EA) inhibited melanin synthesis in a dose-dependent manner in α-melanocyte stimulating hormone (α-MSH)- treated B16/F10 murine melanoma cells. Intracellular tyrosinase activity and melanin contents were suppressed by 45% and 74%, respectively, in response to treatment with 100 μg/mL of R-EA. Examination of protein expression associated with α-MSH-induced melanogenesis showed that tyrosinase related protein (TRP)-1 was inhibited more strongly than tyrosinase, and these results were correlated with stronger inhibition of melanin synthesis than intracellular tyrosinase activity. Taken together, R-EA containing p-coumaric acid and resveratrol could be used as a hypopigmentation agent through suppression of sustained extracellular signal-regulated kinase (ERK) activation via melanogenic induction.
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KEYWORD
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lily bulbs, hypopigmentation, melanin, extracellular signal-regulated kinase
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